Immersion medium supply apparatus, fluorescence spectrometry inspection apparatus, and culture microscope

A fluorescence spectrometry inspection apparatus, comprising:

• a liquid immersion objective which condenses the light from a sample of a fluorescent labeled biological molecule floating in liquid;
• an immersion medium which is filled between the sample and the liquid immersion objective;
• an optical system comprising a photodetector and a fluorescent illumination to detect a magnified image of at least the liquid immersion objective;
• an arithmetic unit which calculates a motion of the sample according to an output from the photodetector;
• a monitor which displays a calculation result by the arithmetic unit;
• a temperature control unit which controls the immersion medium at a predetermined temperature;
• and
• an immersion medium supply unit which supplies the immersion medium objective with the immersion medium controlled at the predetermined temperature by the temperature control unit.

A culture microscope comprising:

• a culture vessel which contains a stage on which an observation sample is placed, a microscope which supports the stage, a liquid immersion objective which is supported by the microscope, and an image pickup unit and optics which capture a magnified image of the observation sample set in the microscope;
• an immersion medium which is filled between the observation sample and the liquid immersion objective;
• a temperature control unit which controls the immersion medium at a predetermined temperature;
• an immersion medium supply unit which supplies the immersion medium objective with the immersion medium controlled at the predetermined temperature by the temperature control unit;
• and
• an warm air circulator which feeds warm air to the culture vessel.