Alpha 1-antitrypsin preparation as well as a method for producing the same
AssigneeBaxter Aktiengesellschaft
InventorsErwin Mattes, H. Peter Matthiessen
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Abstract
A native, chromatographically purified α1-AT preparation having a purity of at least 0.7 PU/mg protein and a relative plasma α1-AT activity of at least 120% is disclosed. The ratio of active to inactive α1-AT is higher than in plasma. Furthermore, a method of producing this preparation is disclosed, as well as the use of a carrier material, e.g. an inorganic carrier material such as hydroxylapatite for separating active α1-AT from inactive α1-AT.
Claims15
A method of preparing a purified biologically active alpha 1-antitrypsin (α1-AT) preparation containing an α1-AT isomer having a pI of between 4.3 and 4.4 comprising:
- providing a starting material containing active and inactive α1-AT, wherein the starting material is a plasma fraction obtained from pooled human plasma;
- providing a hydroxyapatite substrate;
- passing said starting material containing α1-AT over said hydroxyapatite substrate;
- eluting a biologically active α1-AT biologically active α1-AT preparation having a pI of between 4.3 and 4.4;
- and passing said biologically active α1-AT preparation over an anion exchange material in the presence of a detergent.
A method for purifying biologically active α1-AT including α1-AT isomers having pIs of between 4.3 and 4.4 from an alpha 1-AT-containing fraction which is obtainable from a human plasma pool comprising:
- Adjusting the pH of said α1-AT-containing fraction to about 6.5, absorbing said acidified α1-AT-containing fraction onto a chromatographic anion exchanger in the presence of a detergent, and eluting said biologically active α1-AT from said chromatographic anion exchanger including α1-AT isomers having pIs of between 4.3 and 4.4.
Dependencies are inferred from the claim text.