Support with collagen base for tissue engineering and manufacture of biomaterials

A method of in vitro testing of the efficacy of potentially active substance comprising monitoring the effect of said potentially active substance on an artificial skin prepared from a composite collagen support comprising at least one porous collagen layer covered on at least one side with a collagen membrane comprising a collagen film prepared by drying a collagen gel in air separately from the porous collagen layer, said porous collagen layer comprising living fibroblasts cells and said collagen membrane comprising on a surface thereof living cells other than fibroblast;

• and evaluating the monitored effect to determine selection or non selection of said substance as an efficient active substance.

A method of in vitro testing of the efficacy of a potentially active substance comprising monitoring the effect of said potentially active substance on an artificial skin prepared from a tissue engineering support comprising a porous collagen layer prepared from a collagen gel of aquatic origin;

• and a collagen membrane comprising a compressed collagen sponge, wherein said compression is carried out at a pressure of at least about 50 bar, wherein said porous collagen layer is covered on at least one side with said collagen membrane, and wherein at least one of said porous collagen layer and of said collagen membrane comprises living cells selecting from the group consisting of normal living cells, genetically modified living cells and malignant living cells;
• and evaluating the monitored effect to determine selection or non selection of said substance as an efficient active substance.