# US 6,969,603 B2 · Method for isolating DNA

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- Number: US 6,969,603 B2
- Document ID: US6969603B2
- Status: Granted
- Kind: B2
- Application number: 09/984,742
- Filed: 2001-10-31
- Published: 2002-02-28
- Granted: 2005-11-29
- Earliest claimed: 1997-09-22
- Assignees: Riken; Kabushiki Kaisha Dnaform
- Inventors: Yoshihide Hayashizaki; Piero Carninci
- Web page: https://patentgrep.com/patent/US6969603B2

## CPC

- C12N 15/1017: {by filtration, e.g. using filters, frits, membranes}
- C12N 15/1006: {by means of a solid support carrier, e.g. particles, polymers}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

## Claims

### Claim 1

A method for isolating DNA contained in a biological sample, comprising:
combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution of the DNA-containing biological sample, the salt, the cationic surfactant, and the DNA-binding carrier to form a bound DNA-carrier;
separating the DNA-bound carrier from other components;
dissociating the bound DNA from the DNA-binding carrier; and
recovering dissociated DNA.

### Claim 2 (depends on 1)

The method of claim 1, wherein the DNA-containing biological sample comprises blood.

### Claim 3 (depends on 1)

The method of claim 1, wherein the DNA-containing biological sample comprises a whole biological sample.

### Claim 4 (depends on 1)

The method of claim 1, wherein the DNA-containing biological sample is not pretreated by centrifugation.

### Claim 5 (depends on 1)

The method of claim 1, wherein the dissociation of the bound DNA is conducted without phosphate.

### Claim 6 (depends on 1)

The method of claim 1, wherein the DNA-containing biological sample comprises a cell.

### Claim 7 (depends on 1)

The method of claim 1, wherein the DNA-containing biological sample comprises a biological tissue.

### Claim 8

A method for isolating DNA contained in a biological sample, comprising:
including a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite in a solution;
including a DNA-containing biological sample in the solution of the salt, the cationic surfactant and the DNA-binding carrier so that the solution has a salt concentration higher than the DNA precipitation inhibition-initiating concentration so as to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution of the DNA-containing biological sample, the salt, the cationic surfactant, and the DNA-binding carrier to form a bound DNA-carrier;
separating the DNA-bound carrier from other components;
dissociating the bound DNA from the DNA-binding carrier; and
recovering dissociated DNA.

### Claim 9 (depends on 8)

The method of claim 8, wherein the DNA-containing biological sample comprises blood.

### Claim 10 (depends on 8)

The method of claim 8, wherein the DNA-containing biological sample comprises a whole biological sample.

### Claim 11 (depends on 8)

The method of claim 8, wherein the DNA-containing biological sample is not pretreated by centrifugation.

### Claim 12 (depends on 8)

The method of claim 8, wherein the dissociation of the bound DNA is conducted without phosphate.

### Claim 13 (depends on 8)

The method of claim 8, wherein the DNA-containing biological sample comprises a cell.

### Claim 14 (depends on 8)

The method of claim 8, wherein the DNA-containing biological sample comprises a biological tissue.