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US 6,967,239 B1Granted
Method of filtering viruses from a factor VIII solution
AssigneeLaboratoire Francias du Franctionnement et des Biotechnologies
InventorsAbdessatar Chtourou, Michel Nogre, Pierre Porte
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Abstract
Method for preparing a factor VIII solution that is essentially free of viruses and essentially devoid of vWF (von Willebrand factor) and factor VIII-vWF complexes by (a) obtaining a starting factor VIII solution devoid of factor VIII-vWF complexes; and (b) filtering the solution through a hydrophilic virus filter.
Claims13
A method for preparing a factor VIII solution comprising:
- (a) obtaining a starting factor VIII solution comprising factor VIII-von Willebrand factor (factor VIII-vWF) complexes;
- and
- (b) filtering said solution through a hydrophilic virus filter, wherein the virus filter has a mean pore size of 13 to 17 nm and
- wherein the filtering occurs in the presence of CaCl2 ions, at a pressure of less than 0.3 bar and a temperature of about 35±5° C.;
- and
- wherein the filtered solution is free of viruses and devoid of high molecular forms of vWF and factor VIII-vWF complexes.
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Dependencies are inferred from the claim text.