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US 6,964,855 B2Granted
Peptides for detection to Anaplasma phagocytophilum
AssigneeIDEXX Laboratories, Inc.
InventorsThomas Patrick O'Connor, Jr., Ramaswamy Chandrashekar
Life of this patent
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Abstract
The invention provides compositions and methods for the detection and quantification of A. phagocytophilum (formerly known as Ehrlichia equi) antibodies and antibody fragments.
Claims23
A method of detecting antibodies specific for A. phagocytophilum comprising:
- (a) contacting at least one polypeptide consisting essentially of SEQ ID NO:1 and SEQ ID NO:2 with a test sample suspected of comprising antibodies specific for A. phagocytophilum, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for A. phagocytophilum are present in the test sample.
A method of diagnosing an infection in a mammal comprising:
- (a) obtaining a biological sample from a mammal suspected of having an A. phagocytophilum infection;
- (b) contacting at least one polypeptide consisting essentially of SEQ ID NO:1 and SEQ ID NO:2, with the biological sample from (a) under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the mammal has an A. phagocytophilum infection.
1 more element
A method of detecting antibodies specific for A. phagocytophilum comprising:
- contacting a test sample suspected of comprising antibodies specific for A. phagocytophilum with either (i) a polypeptide consisting essentially of SEQ ID NO:3, or (ii) the polypeptide consisting essentially of SEQ ID NO:3 and at least one of a polypeptide consisting essentially of SEQ ID NO:1 and SEQ ID NO:2, wherein the contacting is under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for A. phagocytophilum are present in the test sample.
Dependencies are inferred from the claim text.