Prion protein standard and method of making the same
AssigneeThe Regents of the University of California
InventorStanley B. Prusiner
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Abstract
The invention provides prion protein standards for use as reference materials for prion detection. The standard may be species specific, i.e. the standard is comprised of a preparation for detection of a single strain prion or it may be prepared to allow detection of multiple prion strains simultaneously. The invention also provides methods of preparing the prion protein standards using a group of non-human host mammals which have their genome manipulated with respect to genetic material related to a PrP gene such that the mammals are susceptible to infection with a prion which generally only infects an animal which is genetically diverse from the host.
Claims3
A standardized prion preparation, comprising:
- prions obtained from a plurality of transgenic mouse brains;
- and
- a carrier;
- wherein the preparation comprises prions (a) which infect and cause disease in an animal chosen from a human, a cow, and a sheep, (b) which are prions of a known strain, (c) the prions are present in a known number of infectious units, and further wherein the carrier is different from brain tissue of the animal chosen from a human, a cow and a sheep;
- wherein the prions are uniformly dispersed in the preparation and are produced in a transgenic mouse selected from the group consisting of:
- Tg(HuPrP)/Prnp0/0, Tg(ShePrP)/Prnp0/0, and Tg(BovPrP)[Prnp0/0.
A standardized prion preparation, comprising:
- prions obtained from a plurality of transgenic mouse brains;
- and
- a carrier;
- wherein the preparation comprises prions (a) which infect and cause disease in a human, (b) which are prions of a known strain, (c) the prions are present in a known number of infectious units, and further wherein the carrier is different from brain tissue of a human;
- wherein the prions are uniformly dispersed in the preparation and are produced in a transgenic mouse which is Tg(MHu2M)/Prnp0/0.
A standardized prion preparation, comprising:
- prions obtained from a plurality of mice which are Tg (BovPrP)Prnp0/0;
- and
- a carrier;
- wherein the prions are present in a known number of infectious units infect and cause disease in a cow and are prions of a known strain.
Dependencies are inferred from the claim text.