US 6,960,437 B2 · Nucleic acid amplification utilizing microfluidic devices

Abstract

The present invention provides microfluidic devices and methods using the same in various types of thermal cycling reactions. Certaom devices include a rotary microfluidic channel and a plurality of temperature regions at different locations along the rotary microfluidic channel at which temperature is regulated. Solution can be repeatedly passed through the temperature regions such that the solution is exposed to different temperatures. Other microfluidic devices include an array of reaction chambers formed by intersecting vertical and horizontal flow channels, with the ability to regulate temperature at the reaction chambers. The microfluidic devices can be used to conduct a number of different analyses, including various primer extension reactions and nucleic acid amplification reactions.

Claims48

Dependent claims

A microfluidic device, comprising:

(a) a substrate comprising an elastomeric material;
(b) a flow channel disposed within the substrate, the flow channel
configured such that a sample introduced into the flow channel can be cycled around the flow channel;
and
comprising a plurality of temperature regions at which temperature can be regulated, each temperature region located at a different location along the flow channel;
(c) an inlet in fluid communication with the flow channel via which the sample can be introduced into the flow channel;
and
(d) a temperature controller operatively disposed to regulate temperature within at least one of the plurality of temperature regions.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/118,466Filed 5 Apr 2002 · us provisional application

Priority

6 Apr 2001

Published

US 2003/0008308 A19 Jan 2003

Granted

1 Nov 2005

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12Q 1/68
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
B01F 25/51
Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle: in which the mixture is…
B01F 25/54
Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle: provided with a pump inside…
B01F 33/30
Other mixers; Mixing plants; Combinations of mixers: micromixers

Family

Application60/281,960Us provisional application · filed 6 Apr 2001

Application60/300,516Us provisional application · filed 22 Jun 2001

Application60/334,473Us provisional application · filed 16 Nov 2001

US 2003/0008308 A1Pre-grant publication · published 9 Jan 2003

US 6,960,437 B2This patent

From the US filing history. Foreign family members aren’t listed.

Nucleic acid amplification utilizing microfluidic devices

Life of this patent

Nucleic acid amplification utilizing microfluidic devices

Life of this patent

Abstract

Claims48