Method for scanning microscopy; and scanning microscope
AssigneeLeica Microsystems Heidelberg GmbH
InventorsJohann Engelhardt, Juergen Hoffmann
Life of this patent
Estimating the term…
Abstract
A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.
Claims20
A method for scanning microscopy comprising the steps of:
- generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm;
- choosing a second substantially continuous wavelength spectrum that is arranged spectrally within the first wavelength spectrum;
- selecting light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component;
- and
- scanning a specimen with the light of the second wavelength spectrum.
A scanning microscope comprising:
- a light source that generates an illuminating light beam, for illumination of a specimen, which exhibits at least a first continuous wavelength spectrum whose spectral width is greater than 5 nm, having a detector that detects a detected light beam proceeding from the specimen, having an acoustooptical component with which light of a second substantially continuous wavelength spectrum that is arranged spectrally within the first wavelength spectrum can be selected out of the illuminating light beam, and having a scanning device configured to scan the specimen with the light of the second wavelength spectrum.
A confocal scanning microscope comprising:
- a light source that generates an illuminating light beam, for illumination of a specimen, which exhibits at least a first continuous wavelength spectrum whose spectral width is greater than 5 nm, having a detector that detects a detected light beam proceeding from the specimen, having an acoustooptical component with which light of a second substantially continuous wavelength spectrum that is arranged spectrally within the first wavelength spectrum can be selected out of the illuminating light beam, and having a scanning device configured to scan the specimen with the light of the second wavelength spectrum.
Dependencies are inferred from the claim text.