Method for purifying 5′-protected 2′-deoxypurine nucleosides
AssigneeMitsui Chemicals, Inc.
InventorsHironori Komatsu, Toshiyuki Kouno, Katsutoshi Tsuchiya
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Abstract
A method for efficiently purifying 5′ protected 2′-deoxypurine nucleosides, efficient production of which has previously been difficult. Impurities can be separated by obtaining the 5′ protected 2′-deoxypurine nucleoside as an inclusion crystal including a solvent such as that having a nitrile structure in order to purify the 5′ protected 2′-deoxypurine nucleoside at a high purity. This invention enables synthesis of highly purified protected deoxypurine nucleosides easily on a large scale, which has previously been performed by column chromatography method.
Claims8
A method of purifying a 5′ protected 2′-deoxypurine nucleoside, which comprises the steps of:
- (a) preparing a solution of a compound represented by the following formula (1):
- wherein R1 represents a 4-methoxytrityl group, 4,4′-dimethoxytrityl group or triphenylmethyl group, and B represents a purine group wherein an amino group is protected;
- in a liquid medium comprising a solvent for forming inclusion crystals comprising said solvent and the compound of formula (1);
- (b) forming inclusion crystals comprising said solvent and the compound of formula (1) in said solution by treating said solution alone;
- and
- (c) recovering said inclusion crystals from the liquid medium of the solution.
Dependencies are inferred from the claim text.