Isolation, characterization, cloning and use of a mushroom lectin
AssigneeThe Regents of the University of Michigan
InventorsIrwin J. Goldstein, Harry C. Winter, Robert P. Kruger
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Abstract
The isolation, characterization, cloning and expression of the lectin (agglutinin) from Marasmius oreades (MOA) is described. MOA displays unique carbohydrate binding properties, including blood group B-specific agglutination and preferential binding to Ga1α1,3Ga1-containing sugar epitopes, including but not limited to Ga1α1,3Ga1β1,4G1cNAc. MOA is contemplated as an affinity reagent, a therapeutic in the treatment of antibiotic-induced diarrhea and the field of xenotransplantation. MOA may also serve as a diagnostic reagent, e.g. for malaria.
Claims6
A method of detecting a glycoconjugate, comprising:
- a) providing:
- (i) a composition comprising labeled M. oreades agglutinin, and
- (ii) a sample obtained from a subject;
- and
- b) contacting said sample with said labeled M. oreades agglutinin under conditions such that a glycoconjugate in said sample is detected.
Dependencies are inferred from the claim text.