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US 6,958,318 B2Granted
Recombinant bacterial cells for delivery of PNP to tumor cells
AssigneesThe UAB Research Foundation, Southern Research Institute
InventorsEric J. Sorscher, William B. Parker, William Waud, Vijayakrishna K. Gadi, Leonard L. Bennett, Jr.
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Abstract
The present invention provides a procaryotic host cell stably transformed or transfected by a vector including a DNA sequence encoding for purine nucleoside phosphorylase or hydrolase. The transformed or transfected procaryotic host cell can be used in combination with a purine substrate to treat tumor cells and/or virally infected cells.
Claims5
A method of killing replicating or non-replicating, targeted mammalian cells and bystander cells, comprising the steps of:
- (a) delivering directly to the targeted mammalian cells a purine cleavage enzyme;
- and
- (b) contacting the targeted cells with an effective amount of a substrate for the purine cleavage enzyme, wherein the substrate is non-toxic to mammalian cells and is cleaved by the cleavage enzyme to yield a purine base which is toxic to the targeted mammalian cells and bystander cells, to kill the mammalian cells contacted with the cleavage enzyme and the bystander cells.
Dependencies are inferred from the claim text.