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US 6,958,244 B2Granted
Low-conductivity buffers for magnetic resonance measurements
AssigneeBruker BioSpin GmbH
InventorsVolker Dötsch, Richard S. Withers
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Abstract
An NMR buffer is particularly useful for biomolecules that require a certain amount of salt in the buffer solution. A primary buffer component and a titrating component are selected based on low ion mobility. These selections allow for a lower conductivity buffer without reducing the salt content. As such, a higher sensitivity experiment results.
Claims14
A method of performing an NMR sample analysis, the method comprising:
- providing a deuterated primary buffer component having an ion mobility sufficiently low that, at a concentration of 200 mM, it has a conductivity no greater than 5.0 mS/cm;
- titrating the primary buffer component with a titrating agent that changes the pH of the primary buffer component to be approximately equal to its pKa, the titrating agent having an ion mobility sufficiently low that, after titration, the conductivity of the titrated buffer is no greater than 5.0 mS/cm;
- placing a sample comprising a molecular substance of interest in the titrated buffer;
- and
- conducting an NMR analysis of the sample in the titrated buffer with an NMR spectrometer.
2 more elements
Dependencies are inferred from the claim text.