Variants of Erwinia-type creatinase
AssigneeRoche Diagnostics Operations, Inc.
InventorsZhixin Shao, Rainer Schmuck, Peter Kratzsch, Janet Kenklies, Harald Weisser
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Abstract
The present invention relates to mutated genes encoding variants of an Erwinia-type creatinase (EC 3.5.3.3, alternative name: creatine amidinohydrolase), to the creatinase variants encoded by these genes, and to different applications of these variants of creatinase, particularly to their use for determining the creatinine and/or creatine concentration in a sample.
Claims9
A variant creatinase having creatinase activity comprising at least one amino acid substitution at a position corresponding to an amino acid position of SEQ ID NO:
- 2 selected from the group of positions consisting of N130, M203, I278, I304, and F395, wherein the variant creatinase comprises a sequence with 20 or less adding acid substitutions compared to SEQ ID NO:2.
A variant creatinase having creatinase activity comprising at least two amino acid substitutions at positions corresponding to amino acid positions of SEQ ID NO:
- 2, the positions being selected from the group of positions consisting of N130, M203, I278, I304, and F395, wherein at least one amino acid substitution is at position N130 or I278 and at least one substitution is at position I304 or F395, wherein the variant creatinase comprises a sequence with 20 or less amino acid substitutions compared to SEQ ID NO:2.
Dependencies are inferred from the claim text.