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US 6,958,229 B2Granted
Method for producing highly unsaturated fatty acids and lipid containing same
AssigneeSuntory Limited
InventorsOsamu Suzuki, Kazuhisa Ono, Seiko Shigeta, Tsunehiro Aki, Kengo Akimoto
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Abstract
A method for producing highly unsaturated fatty acids comprising culturing a microorganism, belonging to the genus Mortierella and having resistance to a carbon source, in a medium having a carbon source concentration of at least 4% by weight, and collecting highly unsaturated fatty acids from the cultured products. Culturing the microorganism for about a week gives at least about 7 g/L of highly unsaturated fatty acids.
Claims2
A process for producing arachidonic acid or a lipid containing arachidonic acid comprising the steps of:
- (1) culturing a microrganism belonging to the genus Mortierella and subgenus Mortierella that is strain SAM 2197 FERM BP-6261 , in a medium having a carbon source concentration of at least 4% by weight at the start of culturing and the addition of at least an additional 6% by weight of carbon source during the culturing, thereby forming arachidonic acid or a lipid containing arachidonic acid;
- (2) collecting the cultured cells;
- and
- (3) extracting arachidonic acid or a lipid containing arachidonic acid from the collected cells;
- wherein the microganism produces arachidonic acid of at least about 7 g/L culture medium when cultured in a medium containing at least about 4% carbon source at the start of culturing and the addition of at least an additional 6% by weight of carbon source during the culturing, and at least about 2% nitrogen source at the start of culturing for 5 to 10 days with agitation and aeration.
2 more elements
Dependencies are inferred from the claim text.