Production of feed using a denitrifying methanotrophic bacterial strain
AssigneeE. I. Du Pont de Nemours and Company
InventorsMattheos Koffas, James M. Odom, Andreas Schenzle
Life of this patent
Estimating the term…
Abstract
A high growth methanotrophic bacterial strain capable of growth on a C1 carbon substrate has been isolated and characterized. The strain has the unique ability to utilize both methane and methanol as a sole carbon source and has been demonstrated to possess a functional Embden-Meyerhof carbon flux pathway. The possession of this pathway conveys an energetic advantage to the strain, making it particularly suitable as a production platform for the production of biomass from a C1 carbon source.
Claims1
A method for the production of cellular biomass comprising protein, carbohydrates, and pigment comprising the steps of:
- (a) providing a high growth methanotrophic bacterial strain which:
- (1) grows on a C1 carbon substrate selected from the group consisting of methane and methanol;
- and
- (2) comprises a functional Embden-Meyerhof carbon patahway, said pathway comprising a gene encoding a pyrophosphate dependent phosphofructokinase enzyme as set forth in SEQ ID NO:
- 6;
- (b) contacting the bacterial strain of (a) with a C1 carbon substrate in a suitable medium for a time sufficient to permit the expression and accumulation of the cellular biomass;
- and
- (c) optionally recovering the cellular biomass.
Dependencies are inferred from the claim text.