Detection of herpes simplex virus

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said pair of HSV DNA polymerase primers comprises a first HSV DNA polymerase primer and a second HSV DNA polymerase primer, wherein said second HSV DNA polymerase primer comprises the sequence 5′-CGG GGC GCT CGG CTA AC-3′ (SEQ ID NO:2), wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first HSV DNA polymerase probe comprises the sequence 5′-GCG CAC CAG ATC CAC GCC CTT GAT GAG C-3′ (SEQ ID NO:3);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said second HSV DNA polymerase probe comprises the sequence 5′-CTT GCC CCC GCA GAT GAC GCC-3′ (SEQ ID NO:4);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first HSV DNA polymerase probe comprises the sequence 5′-GTA CAT CGG CGT CAT CTG CGG GGG CAA G-3′ (SEQ ID NO:5);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said second HSV DNA polymerase probe comprises the sequence 5′-T GCT CAT CAA GGG CGT GGA TCT GGT GC-3′ (SEQ ID NO:6);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said K probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said pair of HSV DNA polymerase primers comprises a first HSV DNA polymerase primer and a second HSV DNA polymerase primer, wherein said first HSV DNA polymerase primer comprises the sequence 5′-GCT CGA GTG CGA AAA AAC GTT G-3′ (SEQ ID NO:1), wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of fluorescence resonance energy transfer (FRET) between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said pair of HSV DNA polymerase primers comprises a first HSV DNA polymerase primer and a second HSV DNA polymerase primer, wherein said second HSV DNA polymerase primer comprises the sequence 5′-CGG GGC GCT CGG CTA AC-3′ (SEQ ID NO:2), wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first HSV DNA polymerase probe comprises the sequence 5′-GCG CAC CAG ATC CAC GCC CTT GAT GAG C-3′ (SEQ ID NO:3);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said second HSV DNA polymerase probe comprises the sequence 5′-CTT GCC CCC GCA GAT GAC GCC-3′ (SEQ ID NO:4);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first HSV DNA polymerase probe comprises the sequence 5′-GTA CAT CGG CGT CAT CTG CGG GGG CAA G-3′ (SEQ ID NO:5);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe, wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of HSV DNA polymerase primers to produce an HSV DNA polymerase amplification product if an HSV HSV DNA polymerase nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of HSV DNA polymerase probes, wherein the members of said pair of HSV DNA polymerase probes hybridize to said amplification product within no more than five nucleotides of each other, wherein a first HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a donor fluorescent moiety and wherein a second HSV DNA polymerase probe of said pair of HSV DNA polymerase probes is labeled with a corresponding acceptor fluorescent moiety, wherein said second HSV DNA polymerase probe comprises the sequence 5′-T GCT CAT CAA GGG CGT GGA TCT GGT GC-3′ (SEQ ID NO:6);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first HSV DNA polymerase probe and said acceptor fluorescent moiety of said second HSV DNA polymerase probe,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample;
• said method further comprising:
• performing at least one cycling step, wherein said cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if a TK HSV nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if a TK HSV nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if a TK HSV nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if a TK HSV nucleic acid molecule is present in said sample, wherein said hybridizing step comprises contacting said sample with a pair of TK probes, wherein the members of said pair of TK probes hybridize within no more than five nucleotides of each other, wherein a first TK probe of said pair of TK probes is labeled with a donor fluorescent moiety and wherein a second TK probe of said pair of TK probes is labeled with a corresponding acceptor fluorescent moiety, wherein said first TK probe comprises the sequence 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9), wherein said second TK probe comprises the sequence 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety of said first TK probe and said acceptor fluorescent moiety of said second TK probe upon hybridization of said pair of TK probes to said targets,
• wherein the presence of FRET is indicative of the presence of HSV in said biological sample, and wherein the absence of FRET is indicative of the absence of HSV in said biological sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, wherein said hybridizing step comprises contacting said sample with a TK probe, wherein said TK probe is labeled with a donor fluorescent moiety and a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety and said acceptor fluorescent moiety of said TK probe,
• wherein the presence or absence of FRET is indicative of the presence or absence of HSV in said sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a TK probe, wherein said TK probe is labeled with a donor fluorescent moiety and a corresponding acceptor fluorescent moiety;
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety and said acceptor fluorescent moiety of said TK probe,
• wherein the presence or absence of FRET is indicative of the presence or absence of HSV in said sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequences 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7) wherein R is A or G, and wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8) wherein M is A or C, wherein said hybridizing step comprises contacting said sample with a TK probe, wherein said TK probe is labeled with a donor fluorescent moiety and a corresponding acceptor fluorescent moiety, wherein said TK probe comprises a sequence selected from the group consisting of 5′-CGC GCG ACG ATA TCG TCT ACG TAC-3′ (SEQ ID NO:9) and 5′-CGA GCC GAT GAC TTA CTG GCA GGT G-3′ (SEQ ID NO:10);
• and
• detecting the presence or absence of FRET between said donor fluorescent moiety and said acceptor fluorescent moiety of said TK probe,
• wherein the presence or absence of FRET is indicative of the presence or absence of HSV in said sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a dye-binding step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said first TK primer comprises the sequence 5′-CAC GCT RCT GCG GGT TTA TAT AGA-3′ (SEQ ID NO:7), wherein R is A or G, wherein said dye-binding step comprises contacting said TK amplification product with a nucleic acid binding dye;
• and
• detecting the presence or absence of binding of said nucleic acid binding dye to said amplification product,
• wherein the presence of binding is indicative of the presence of HSV in said sample, and wherein the absence of binding is indicative of the absence of HSV in said sample.

A method for detecting the presence or absence of HSV in a biological sample from an individual, said method comprising:

• performing at least one cycling step, wherein a cycling step comprises an amplifying step and a dye-binding step, wherein said amplifying step comprises contacting said sample with a pair of TK primers to produce a TK amplification product if an HSV TK nucleic acid molecule is present in said sample, wherein said pair of TK primers comprises a first TK primer and a second TK primer, wherein said second TK primer comprises the sequence 5′-TTG TTA TCT GGG CGC TMG TCA TT-3′ (SEQ ID NO:8), wherein M is A or C, wherein said dye-binding step comprises contacting said TK amplification product with a nucleic acid binding dye;
• and
• detecting the presence or absence of binding of said nucleic acid binding dye to said amplification product,
• wherein the presence of binding is indicative of the presence of HSV in said sample, and wherein the absence of binding is indicative of the absence of HSV in said sample.