Linkage of agents to body tissue using microparticles and transglutaminase
AssigneePericor Science, Inc.
InventorsHoward Green, Bruce J. Compton, George D. Corey, Philippe Djian
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Abstract
Methods, products and kits are provided for attaching agents to a body tissue surface via microparticles using endogenous or exogenous transglutaminase. The microparticles have surface available transglutaminase substrate reactive groups. In an embodiment, the groups are part of a polymer containing at least two contiguous linked lysines or at least three contiguous linked glutamines.
Claims39
A composition comprising
- a microparticle comprising an active agent and a polymer having transglutaminase substrate reactive groups, wherein the transglutaminase substrate reactive groups are surface available, and the polymer comprises a polymer of amino acids having at least three contiguous linked glutamines.
A composition comprising
- a microparticle comprising an active agent and a polymer having transglutaminase substrate reactive groups, wherein the microparticle is non-biodegradable, and the transglutaminase substrate reactive groups are surface available, and the polymer comprises a polymer of amino acids having at least three contiguous linked lysines.
A method of treating a subject to attach microparticles to a body tissue of the subject comprising
- contacting the body tissue in the presence of endogenous or exogenous transglutaminase with microparticles having surface available transglutaminase substrate reactive groups in an amount sufficient to attach the microparticles to the body tissue in the presence of the transglutaminase,
- allowing the microparticles to remain in contact with the body tissue for a time sufficient to permit a layer of microparticles to covalently attach to the body tissue,
- wherein the transglutaminase substrate reactive groups are part of a polymer, and
- wherein the polymer comprises a polymer selected from the group consisting of polymers containing:
- (a) at least two contiguous linked lysines,
- (b) at least three contiguous linked lysines,
- (c) at least four contiguous linked lysines, and
- (d) at least five contiguous linked lysines.
A method of treating a subject to attach microparticles to a body tissue of the subject comprising
- contacting the body tissue in the presence of endogenous or exogenous transglutaminase with microparticles having surface available transglutaminase substrate reactive groups in an amount sufficient to attach the microparticles to the body tissue in the presence of the transglutaminase,
- allowing the microparticles to remain in contact with the body tissue for a time sufficient to permit a layer of microparticles to covalently attach to the body tissue,
- wherein the transglutaminase substrate reactive groups are part of a polymer, and
- wherein the polymer comprises a polymer selected from the group consisting of polymers containing:
- (a) at least five contiguous linked glutamines,
- (b) at least ten contiguous linked glutamines,
- (c) at least fifteen contiguous linked glutamines, and
- (d) at least twenty contiguous linked glutamines.
Dependencies are inferred from the claim text.