Mammalian haemoglobin compatible with blood plasma, cross-linked and conjugated with polyalkylene oxides as artificial medical oxygen carriers, production and use thereof
AssigneeSanguibiotech AG
InventorWolfgang Barnikol
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Abstract
The invention relates to covalently cross-linked mammalian hemoglobins, to which polyalkylene oxides are covalently linked. Such hemoglobins are surprisingly compatible with proteins of human and animal plasma, under all the possible conditions within the vascular system of the body. The invention furthermore relates to the production of these cross-linked hemoglobins linked with polyalkylene oxides, as well as to their use as artificial intravasal oxygen carriers in the human or animal organism or in individual organs.
Claims17
Hemoglobin derivative compatible with human and non-human animal plasma, comprising hemoglobin crosslinked by means of a crosslinker for proteins and a polyalkylene oxide covalently linked to the hemoglobin, with polyalkylene oxides being excluded as crosslinker and no reaction with diaspirin taking place, said crosslinker being a bifunctional crosslinker for proteins, selected from the group consisting of butane diepoxide, divinyl sulfone, a diisocyanate, a di-N-hydroxysuccinimidyl ester, a diimido ester and a dialdehyde.
Method for the preparation of hemoglobin derivatives as synthetic oxygen carriers which are compatible with human and non-human animal blood plasma, comprising crosslinking the hemoglobin with a crosslinker for proteins and covalently linking a polyalkylene oxide to the hemoglobin, the crosslinked hemoglobin having a molecular weight from 50,000 to 10,000,000 g/mol, polyalkylene oxides as crosslinkers being excluded and no reaction with diaspirin takes place.
Dependencies are inferred from the claim text.