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AssigneeEmory University
InventorsJoseph M. Kinkade, Jr., Raymond Shapira, Peter E. Jensen, Ngoc-Anh Le, Jan Pohl, W. Virgil Brown
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This invention related generally to methods of detecting and quantifying biomarkers of oxidative stress in proteins. The biomarker may be any amino acid that has undergone oxidation (or other modification, e.g. chloro-tyrosine, dityrosine). Emphasis is given herein on oxidized sulfur- or selenium-containing amino acids (SSAA). The biomarker of oxidative stress in proteins may be detected with an antibody that binds to oxidized amino acids, specifically oxidized sulfur- or selenium-containing amino acids. The antibody may be monoclonal or polyclonal. The presence of biomarker or amount of biomarker present in a sample may be used to aid in assessing the efficacy of environmental, nutritional and therapeutic interventions, among other uses.
An immunoassay method to detect a biomarker of oxidative stress in a biological sample utilizing an antibody which binds said biomarker of oxidative stress, said method comprising the steps of:
The method of clam 12 wherein said monoclonal antibody detects biomarkers of oxidative stress in any protein containing said biomarkers.
A method of detecting an oxidized sulfur- or selenium-containing amino acid in a biological sample utilizing an antibody which binds a protein, peptide or proteineaceous aggregate which comprises an oxidized sulfur- or selenium-containing amino acid, said method comprising the steps of:
A method for detecting the presence of oxidative stress in an organism, said method comprising detecting the presence of an antibody that binds an analyte comprising an oxidized sulfur- or selenium containing amino acid, whereby the presence of said antibody is indicative of the presence of oxidative stress in said organism.
Dependencies are inferred from the claim text.