Method of detecting the presence of a liquid in a mix
AssigneeCIS Bio International
InventorsOlivier Pible, Emmanuel Bois
Life of this patent
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Abstract
The invention relates to a method of detecting the presence of a liquid introduced into a mix of constituents. The method comprises the following steps: excitation of the mix by a light signal in the absence of the liquid to be detected; measurement of a fluorescence signal over a time Δt characteristic of the liquid to be detected, so as to produce a reference quantity Ro; introduction of an amount of liquid to be detected in the mix; excitation of the mix by a light signal; measurement of a fluorescence signal over a time Δt so as to produce a quantity R; comparison of the quantity R with a quantity k×Ro. The invention applies to the detection of serum pipetting errors within the context of bioassays, especially immunological tests.
Claims20
A method of detecting the presence of a liquid in a mix, comprising:
- producing a first mix not containing the liquid to be detected;
- exciting the first mix by a light signal of wavelength λ;
- measuring a fluorescence signal S1 emitted by the first mix over a time Δt close to a lifetime τ1 characteristic of the liquid to be detected, so as to produce a reference quantity Ro which is a function of the measured signal S1;
- producing the mix from constituents identical to the constituents making up the first mix and from an amount of the liquid to be detected;
- exciting the mix by a light signal of wavelength λ;
- measuring a fluorescence signal S11 emitted by the mix over the time Δt close to the lifetime τ1 so as to produce a quantity R which is a function of the measured signal S11;
- and
- comparing the quantity R with a quantity k×Ro, wherein k is a coefficient that takes into account the statistical fluctuations of R.
A method of detecting the presence of a liquid in a mix, comprising steps for:
- production (1) of a first mix not containing the liquid to be detected;
- excitation (2) of the first mix by a light signal of wavelength λ;
- measurement (3) of a fluorescence signal S1 emitted by the first mix over a time At close to a lifetime τcharacteristic of the liquid to be detected, so as to produce (4) a reference quantity Ro which is a function of the measured signal S1;
- production (5, 10) of the mix from constituents identical to the constituents making up the first mix and from an amount of the liquid to be detected;
- excitation (6) of the mix by a light signal of wavelength λ;
- measurement (7) of a fluorescence signal S11 emitted by the mix over the time Δt close to the lifetime ri so as to produce (8) a quantity R which is a function of the measured signal S11;
- and
- comparison (9) of the quantity R with a quantity k×Ro, wherein k is a coefficient that takes into account the statistical fluctuations of R.
Dependencies are inferred from the claim text.