Processes and agents for detecting Listerias
AssigneeMerck Patent Gesellschaft mit beschrankter
InventorsPeter Schubert, Siegfried Neumann, Martina Pawelzik, Winfried Linxweiler, Christa Burger, Andreas Bubert, Werner Goebel, Stefan Köhler
Life of this patent
Estimating the term…
Abstract
The invention relates to agents and processes for detecting bacteria of the genus Listeria, in particular L. monocytogenes. The agents according to the invention include primers whose sequence is selected from the iap gene of L. monocytogenes. In addition, the agents according to the invention include peptides whose sequence is selected from the p60 protein and which are suitable for producing specific antibodies for the immunological detection of L. monocytogenes.
Claims17
An isolated antibody which specifically binds to an epitope selected from the group consisting of one of the following peptides:
- SEQ ID NO:
- 17, 20, 26, 29, 30 and 31 of the p60 protein from pathogenic Listeria monocytogenes and wherein said antibody does not react with p60 protein from other non-pathogenic Listeria species.
An isolated antibody which can be prepared by immunizing an experimental animal with a peptide selected from the group consisting of one of the following peptides:
- SEQ ID NO:
- 17, 20, 26, 29, 30 and 31, or with an immunogenic conjugate which comprises a peptide selected from the group consisting of one of the following peptides:
- SEQ ID NO:
- 17, 20, 26, 29, 30 and 31, wherein said antibody specifically binds to the p60 protein from pathogenic Listerina monocytogenes and does not react with p60 protein from other non-pathogenic Listeria species.
Dependencies are inferred from the claim text.