Methods and reagents for quantitation of cell-surface molecule expression on peripheral blood cells
AssigneeBecton, Dickinson and Company
InventorKenneth A. Davis
Life of this patent
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Abstract
Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.
Claims9
A composition for flow cytometric measurement of a cell-surface molecule on human peripheral blood cells, wherein said cell-surface molecule is characterized in that a fluorescent signal measured following staining with a fluorescently labeled antibody specific for said cell-surface molecule in the absence of a lysosomotropic amine depends on the time of incubation with said antibody, said composition comprising:
- a fluorophore-conjugated antibody specific for said cell-surface molecule, and
- a lysosomotropic amine.
Dependencies are inferred from the claim text.