Porous coatings bearing ligand arrays and use thereof
AssigneeSyntrix Biochip, Inc.
InventorJohn A. Zebala
Life of this patent
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Abstract
Articles comprising substantially uniform porous coatings, which may be photopatterned, are provided. The use of such porous coatings increases the surface density of attached compounds within, for example, ligand arrays prepared by methods such as regionally selective solid-phase chemical synthesis. Arrays prepared using the porous coatings may be used within a variety of diagnostic and drug discovery assays.
Claims155
A coated article comprising a substrate having a continuous porous coating of substantially uniform thickness, wherein the coating comprises a gelled network of particles, wherein the porous coating has two or more different compounds attached thereto, and wherein the compounds are attached at known discrete full thickness volumes, each occupying an area on the substrate of less than 1,000.000 μm2.
A method for making a coated article with two or more compounds attached thereto, comprising the steps of:
- (a) applying to a substrate a substantially uniform layer of a solution comprising metal oxide particles dispersed in a volatile liquid;
- (b) evaporating the volatile liquid from the layer, forming a gelled network of metal oxide particles on the substrate, wherein the gelled network forms a porous coating ranging from 0.05 to 25 microns thick;
- and
- (c) attaching two or more compounds to discrete known regions of the porous coating, wherein the compounds are attached at known discrete full thickness volumes, each occupying an area on the substrate of less than 1,000,000 μm2, and therefrom generating a coated article comprising a substrate having a porous coating with two or more compounds attached thereto.
A coated article comprising a substrate having at least two discrete known regions with continuous porous coatings, wherein each coating has a substantially uniform thickness and comprises a gelled network of particles, and wherein each porous coating has at least one compound attached thereto, and wherein a different compound is attached to each of the porous coatings.
A method of making a coated article comprising a substrate and at least two separate porous coatings, comprising the steps of:
- (a) applying to a substrate a substantially uniform layer of a solution comprising metal oxide particles dispersed in a volatile liquid;
- (b) evaporating the volatile liquid from the layer, forming a gelled network of metal oxide particles on the substrate, wherein the gelled network forms a porous coating ranging from 0.05 to 25 microns thick;
- (c) covering the porous coating with a layer of photoresist comprising a base soluble component;
- (d) irradiating the photoresist, such that a first region of photoresist is rendered substantially removable with an aqueous alkaline developer, and such that a second region is not so removable;
- (e) contacting at least the first region with an aqueous alkaline developer to remove at least the first region of photoresist and porous coating underlying the first region, without substantially removing the second region of photoresist or porous coating underlying the second region;
- (f) removing remaining photoresist with an organic solvent, resulting in separate porous coatings on discrete regions of the substrate;
- and
- (g) attaching one or more compounds to each of the separate porous coatings, and therefrom generating a substrate having at least two separate porous coatings, wherein each coating has a substantially uniform thickness and comprises a continuous gelled network of metal oxide particles and polymers of a hydrolyzed metal alkoxide, and wherein a different compound is attached to each of the porous coatings.
A coated article comprising a substrate having a plurality of continuous porous coatings thereon of substantially uniform thickness, wherein each of the porous coatings comprises a continuous gelled network of metal oxide particles and polymers of hydrolyzed metal alkoxide, and wherein each of the porous coatings has a surface area measuring greater than 50 meters2/g.
A coated article comprising a substrate having at least five separate distinct porous coatings per square centimeter, wherein each coating is continuous and has a substantially uniform thickness and comprises a continuous gelled network of particles, and wherein each of the separate porous coatings occupies an area on the substrate of less than about 1,000,000 μm2.
A coated article comprising a substrate having at least two discrete known regions with continuous porous coatings, wherein each coating has a substantially uniform thickness and comprises a gelled network of particles, and wherein each porous coating has at least one compound attached thereto, and wherein each of the separate porous coating occupies an area on the substrate of less than about 10,000 μm2.
A coated article comprising a substrate having at least two discrete known regions with continuous porous coatings, wherein each coating has a substantially uniform thickness and comprises a gelled network of particles, and wherein each porous coating has at least one compound attached thereto, and wherein the porous coatings have a surface area measuring greater than 50 meters 2/g.
Dependencies are inferred from the claim text.