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US 6,949,632 B2Granted
Fluorogenic substrates
AssigneeChromagen, Inc.
InventorsMichael J. Conrad, Liyan He
Life of this patent
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Abstract
The subject invention provides compounds useful as fluorogenic substrates for the hydrolytic enzymes. Upon hydrolysis of the hydrolyzable group, a halo-pyrene substituted molecule is developed which is highly fluorescent, water soluble and exhibits several desirable characteristics, including a large Stokes' shift.
Claims6
A fluorogenic substrate molecule having the following characteristics:
- (a) said molecule re-emits at least about 70% of the energy of the incident radiation that is used to excite the molecule;
- (b) the molecule emits radiation at a wavelength that is at least about 25 nm from the wavelength of radiation used to excite the molecule;
- and
- (c) the molecule is soluble and stable in water;
- wherein said molecule has the following structure:
- and salts thereof, wherein:
- R1 is selected from the group consisting of phospate, —OH, sulfonamide, and substrates for hydrolytic enzymes;
- and
- R2, R3, and R4 are, independently, selected from the group consisting of SO3, NR5R6, SO2NR5R6, and halides;
- wherein R5 and R6 are, independently, selected from the group consisting of H, and C1-6;
- provided that at least one of R2, R3, or R4 is a halide.
8 more elements
Dependencies are inferred from the claim text.