Bacillus thuringiensis cryET33 and cryET34 compositions and uses therefor
AssigneeMonsanto Technology LLC
InventorsWilliam P. Donovan, Judith C. Donovan, Annette C. Slaney
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AssigneeMonsanto Technology LLC
InventorsWilliam P. Donovan, Judith C. Donovan, Annette C. Slaney
Estimating the term…
Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
A purified antibody that binds to a CryET34 peptide, wherein said CryET34 peptide is SEQ ID NO:4.
A purified antibody generated by using a peptide according to SEQ ID NO:4 as an immunogen.
A purified antibody produced by a hybridoma, wherein a recombinant CryET34 peptide according to SEQ ID NO:4, is used to generate the hybridoma producing said antibody.
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