Nucleotide sequences which code for the menE gene
AssigneeDegussa AG
InventorsMike Farwick, Klaus Huthmacher, Achim Marx, Walter Pfefferle
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AssigneeDegussa AG
InventorsMike Farwick, Klaus Huthmacher, Achim Marx, Walter Pfefferle
Estimating the term…
The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the menE gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
An isolated nucleic acid comprising a nucleotide sequence selected from the group consisting of:
An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:2, wherein said polypeptide has O-succinylbenzoic acid CoA ligase activity.
An isolated nucleic acid consisting of SEQ ID NO:
An isolated nucleic acid consisting of a fragment of at least 40 consecutive nucleotides of SEQ ID NO:1 or the full complement thereof, wherein said isolated nucleic acid is a probe in a hybridization reaction to detect an isolate nucleic acid that is at least 90% identical to that of SEQ ID NO:
Dependencies are inferred from the claim text.