Temperature-sensitive and cold-adapted human parainfluenza virus type 2 (HPIV-2) and vaccines based on such virus
AssigneeSt. Louis University
InventorsRobert B. Belshe, Frances K. Newman
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated, attenuated viral strains of human parainfluenza virus 2 (HPIV-2), which are useful in live vaccine preparations. These strains exhibit a temperature sensitive and cold adapted phenotype useful for stimulating a protective immune response in an inoculated mammal without producing severe symptoms.
Claims37
An isolated, attenuated viral strain of human parainfluenza virus 2, wherein the viral strain is temperature sensitive and cold adapted.
A method of producing an isolated, attenuated viral strain of human parainfluenza virus 2, wherein the viral strain is temperature sensitive and cold adapted, the method comprising the steps of:
- (a) culturing in vitro a wild type human parainfluenza virus 2 (HPIV-2);
- (b) cold passaging said virus at a first temperature;
- (c) selecting viruses that grow at the first temperature;
- (d) cold passaging the selected viruses at a second temperature;
- the second temperature being less than the first;
- and
- e) selecting viruses that grow at the second temperature.
Dependencies are inferred from the claim text.