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US 6,942,770 B2Granted
Disposable sub-microliter volume biosensor with enhanced sample inlet
AssigneeNova Biomedical Corporation
InventorsXiaohua Cai, Handani Winarta, Andy Vo, Chung Chang Young
Life of this patent
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Abstract
A disposable electrode strip for testing a fluid sample including a laminated strip with a first and second end, a vent, an open path for receiving a fluid sample of less than one microliter beginning from the first end and connecting to the vent, a working electrode, a reference electrode and a pseudo-working electrode embedded in the laminated strip within the open path and proximate to the first end, a reagent matrix coextensive within the open path and covering the three electrodes, and conductive contacts located at the second end of the laminated strip.
Claims47
A disposable electrode strip for testing a fluid sample comprising:
- a laminated strip having a first strip end, a second strip end and a vent opening spaced from said first strip end, said laminated strip comprising a base layer having a conductive layer disposed thereon, said conductive layer having scribe lines delineated thereon and forming three electrode paths, a channel forming layer carried on said base layer, and a cover having an inlet notch at said first strip end;
- an enclosed channel between said first strip end and said vent opening, said enclosed channel sized to hold a volume of said fluid sample less than one microliter;
- a reagent matrix containing at least an enzyme, a stabilizer, wherein said stabilizer is a dissolvable polyalkylene glycol, and a redox mediator disposed on said base layer in said enclosed channel;
- conductive contacts at said second strip end and insulated from said enclosed channel.
1 more element
A disposable electrode strip for detecting or measuring the concentration of an analyte in a fluid sample, said electrode strip comprising:
- an insulating base strip having a first base end and a second base end;
- a conductive layer disposed on one side of said base strip, said conductive layer having a pattern scribed into said conductive layer, said pattern delineating three electrically-distinct conductive paths insulated from each other;
- a middle insulator sized smaller thin said insulating base strip and overlaying a substantial portion of said conductive layer, said middle insulator having a cutout portion spaced from said first base end, said cutout portion exposing a limited area of said three conductive paths;
- an electrode material comprising an enzyme, a redox mediator, a stabilizer wherein said stabilizer is a dissolvable polyalkylene glycol, a binder, a surfactant, and a buffer, said electrode material being disposed in said cutout portion;
- and
- a covering insulator sized to fit over and be coextensive with said middle insulator creating a sample fluid channel, said covering insulator having an inlet notch at a first covering insulator end, said first covering insulator end being coextensive with said first base end, and a covering insulator aperture spaced from said first base end and configured to expose at least a small portion of said cutout portion of said middle insulator.
3 more elements
A laminated biosensor strip comprising:
- a base layer with an electrode end and an electrical contact end, said base layer having an electrically conductive coating on one side wherein said conductive coating has a plurality of scribed lines delineating a first conductive path having an L-shape, a second conductive path having a mirror-image L-shape wherein the L-shape ends of said first and second conductive paths are adjacent to each other, and a third conductive path which is shorter than either of said first and second conductive paths, has a linear, elongated shape wherein said L-shaped ends of said first and second conductive paths and the end of said third conductive path are aligned with each other along the central axis of said base layer;
- a channel forming layer disposed over said base layer, said channel forming layer having a U-shaped end portion defining a central elongated channel sized to expose a portion of each of said L-shaped ends of said first and second conductive paths and a portion of said third conductive path, said channel forming layer being shorter in length than said base layer wherein a portion of each of said three conductive paths is exposed at said electrical contact end;
- a reagent material having at least a redox mediator, a stabilizer, wherein said stabilizer is a dissolvable polyalkylene glycol, and an enzyme disposed within said U-shaped end portion and covering the exposed portions of said first, second and third conductive paths;
- and
- a cover with a vent, said cover disposed on and coextensive with said channel forming layer, said cover, said channel forming layer and said base layer forming a capillary channel with an inlet at one end and communicating with said vent at the other end.
2 more elements
A disposable electrode strip for testing a fluid sample comprising:
- a laminated strip having a first strip end, a second strip end and a vent opening spaced from said first strip end, said laminated strip comprising a base layer having a conductive layer disposed thereon, said conductive layer having scribe lines delineated thereon and forming at least two electrode paths, a channel forming layer carried on said base layer, and a cover having an inlet notch at said first strip end;
- an enclosed channel between said first strip end and said vent opening, said enclosed channel sized to hold a volume of said fluid sample less than one microliter;
- a reagent matrix containing at least an enzyme, a stabilizer, wherein said stabilizer is a dissolvable polyalkylene glycol, and a redox mediator disposed on said base layer in said enclosed channel;
- conductive contacts at said second strip end and insulated from said enclosed channel.
1 more element
Dependencies are inferred from the claim text.