Nucleic acids encoding costimulatory molecule B7-4
AssigneeDana-Farber Cancer Institute, Inc.
InventorsGordon Freeman, Vassiliki Boussiotis, Tatyana Chernova, Nelly Malenkovich
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Abstract
The invention provides isolated nucleic acids molecules, designated B7-4 nucleic acid molecules, which encode novel B7-4 polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing B7-4 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a B7-4 gene has been introduced or disrupted. The invention still further provides isolated B7-4 proteins, fusion proteins, antigenic peptides and anti-B7-4 antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
Claims64
An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:
- 1.
An isolated nucleic acid molecule encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2.
An isolated nucleic acid molecule comprising nucleotides 107-766 of SEQ ID NO:
- 3.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding amino acids 19-238 of SEQ ID NO:
- 4.
An isolated nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:
- 3.
An isolated nucleic acid molecule encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 4.
An isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the complement of the nucleic acid molecule of SEQ ID NO:3 over its full length under conditions of incubation at 45° C. in 6× sodium chloride/sodium citrate (SSC), followed by washing in 0.2×SSC, 0.1% SDS, at 50-60° C., wherein the nucleic acid molecule encodes a polypeptide that costimulates T cell proliferation in vitro when the polypeptide is present on a first surface and an antigen or a polyclonal activator that transmits an activating signal via the T-cell receptor is present on a second, different surface.
An isolated nucleic acid molecule comprising a nucleotide sequence which hybridizes to the complement of the nucleic acid molecule of SEQ ID NO:1 over its full length under conditions of incubation at 45° C. in 6× sodium chloride/sodium citrate (SSC), followed by washing in 0.2×SSC, 0.1% SDS, at 50-60° C., wherein the nucleic acid molecule encodes a polypeptide that costimulates T cell proliferation in vitro when the polypeptide is present on a first surface and an antigen or a polyclonal activator that transmits an activating signal via the T-cell receptor is present on a second, different surface.
An isolated nucleic acid molecule comprising a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO:
- 3, and that encodes a polypeptide that costimulates T cell proliferation in vitro when the polypeptide is present on a first surface and an antigen or a polyclonal activator that transmits an activating signal via the T-cell receptor is present on a second, different surface.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO:
- 4, wherein the polypeptide costimulates T cell proliferation in vitro when the polypeptide is present on a first surface and an antigen or a polyclonal activator that transmits an activating signal via the T-cell receptor is present on a second, different surface.
An isolated nucleic acid molecule which is a fragment of SEQ ID NO:1 or SEQ ID NO:3, or the complement thereof, wherein the fragment consists of at least 15 contiguous nucleotides of a nucleotide sequence selected from the group consisting of:
- (a) nucleotides 1-319 of SEQ ID NO:
- 1;
- (b) nucleotides 53-922 of SEQ ID NO:
- 3;
- (c) nucleotides 1-314 of SEQ ID NO:
- 3;
- and
- (d) nucleotides 955-1285 of SEQ ID NO:
- 3.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding the amino acids 19-245 of SEQ ID NO:2.
An isolated nucleic acid molecule comprising nucleotides 107-733 of SEQ ID NO:
- 3.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding amino acids 19-227 of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.