Recombinant cell clones having increased stability and methods of making and using the same
AssigneeBaxter Aktiengesellschaft
InventorsManfred Reiter, Wolfgang Mundt, Friedrich Dorner
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Abstract
Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
Claims27
A recombinant mammalian cell clone stable in serum- and protein-free medium for at least 40 generations and expressing a recombinant product.
A cell culture comprising at least 90% of stable recombinant mammalian cells, said stable recombinant cells being stable for more than 40 generations under serum- and protein-free conditions and expressing recombinant product.
A cell culture comprising more than 95% of stable recombinant mammalian cells, said stable recombinant cells being stable for more than 40 generations under serum- and protein-free conditions and expressing recombinant product.
A method for preparing a recombinant polypeptide in a cell culture, comprising:
- multiplying a stable mammalian cell clone containing a sequence encoding the recombinant polypeptide in a serum- and protein-free medium-comprising a mixture of amino acids containing L-asparagine, L-cysteine, L-cystine, L-proline, L-tryptophan and L-glutamine from an original recombinant clone,
- preparing a cell culture containing the stable cell clone in a bioreactor,
- harvesting the recombinant polypeptide from the culture supernatant.
Dependencies are inferred from the claim text.