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OwnerDon J. Chandler, Van S. Chandler +3inventors
InventorsDon J. Chandler, Van S. Chandler, Beth A. Lambert, Janet J. Reber, Stacie L. Phipps
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An improved method of making a series of bead or microsphere or particle populations characterized by subtle variation in a proportion or ratio of at least two fluorescent dyes distributed within a single bead of each population is provided. These beads, when excited by a single excitation light source are capable of giving off several fluorescent signals simultaneously. A set containing as many as 64 distinct populations of multicolored, fluorescent beads is provided and when combined with analytical reagents bound to the surface of such beads is extremely useful for multiplexed analysis of a plurality of analytes in a single sample. Thus, methods of staining polymeric particles, the particles themselves, and methods of using such particles are claimed.
A population of preformed polymeric microspheres stained using a dye bath solution, wherein the dye bath solution comprises predetermined concentrations of at least two fluorescent dyes, wherein each the microspheres upon excitation emit at least two distinct fluorescent signals corresponding to the at least two fluorescent dyes, wherein an intensity of each of the at least two emitted signal is proportional to an amount of its corresponding dye in microsphere, and wherein a target fluorescence intensity of said stained preformed polymeric microspheres has been used to determine the concentrations of the at least two fluorescent dyes in the dye bath solution.
A population of fluorescently dyed polymeric microspheres dyed by a process comprising the steps of:
A population of polymeric microspheres dyed with two or more fluorescent dyes, said population of polymeric microspheres being dyed using a process comprising the steps of:
A population of preformed polymeric microspheres dyed using a fluorochrome bath solution, wherein the fluorochrome bath solution comprises predetermined concentrations of at least two fluorochromes, wherein each the microspheres upon excitation emit at least two distinct fluorescent signals corresponding to the at least two fluorochromes, wherein an intensity of the at least two emitted signals is proportional to an amount of its corresponding fluorochrome in the microsphere, and wherein a target fluorescence intensity of said dyed preformed polymeric microspheres has been used to determine the concentrations of the at least two fluorochromes in the fluorochrome bath solution.
A population of dyed polymeric microspheres dyed by a process comprising the steps of:
Dependencies are inferred from the claim text.