Orotidine-5′-phosphate decarboxylase-gene, gene construct containing said gene and the utilization thereof
AssigneeBASF SE
InventorsMarkus Pompejus, Jose Luis Revuelta Doval, Maria Angeles Santos Garcia
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Abstract
The invention relates to an orotidine-5′-phosphate decarboxylase gene having the sequence of SEQ ID NO: 1 or its homologs, to a gene construct comprising this gene or its homologs, and to its use. The invention additionally relates to vectors or organisms comprising an orotidine-5′-phosphate decarboxylases gene having the sequence SEQ ID NO: 1 or its homologs. The invention further relates to a process for producing uracil-auxotrophic microorganisms and to a process for inserting DNA into uracil-auxotrophic microorganisms.
Claims15
An isolated orotidine-5′-phosphate decarboxylase gene having the sequence SEQ ID NO:
- 1 and homologs of said sequence having at least 90% homology on the derived amino acid level.
An orotidine-5′-phosphate decarboxylase gene having the sequence SEQ ID NO:
- 1 which is isolated from Ashbya gossypii.
A process for inserting DNA into microorganisms, which comprises inserting a vector which comprises an intact orotidine-5′-phosphate decarboxylase gene having the sequence SEQ ID NO:
- 1 or its homologs isolated from microorganisms which have at least 90% homology with the sequence as depicted in SEQ ID NO:
- 1 together with at least one other nucleic acid sequence, into a microorganism which is deficient in orotidine-5′-phosphate decarboxylase enzymatic activity and cultivating this microorganism on or in a culture medium without uracil.
Dependencies are inferred from the claim text.