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US 6,924,151 B2Granted
Stable factor VIII/vWF-complex
AssigneeBaxter Aktiengesellschaft
InventorsBernhard Fischer, Artur Mitterer, Friedrich Dorner, Johann Eibl
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Abstract
There are disclosed a stable factor VIII/vWF-complex, particularly comprising high-molecular vWF multimers, being free from low-molecular vWF molecules and from proteolytic vWF degradation products, as well as a method of producing this complex.
Claims13
A method of recovering stable Factor VIII/von Willebrand Factor (vWF)-complex from a protein solution contaminated with other proteins, wherein the method comprises
- binding the Factor VIII/vWF-complex contained in the protein solution to an anion exchanger;
- selectively eluting the other proteins with an eluting agent containing a calcium salt and an elution salt, wherein the elution salt is present at a concentration of no more than 200 mM, and
- subsequently recovering Factor VIII/vWF-complex from the anion exchanger using a buffer having an elution salt at a concentration of 200 to 400 mM, wherein the buffer is without a calcium salt.
Dependencies are inferred from the claim text.