# US 6,917,423 B2 · Method for detection of pathogenic microorganisms > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 6,917,423 B2 - Document ID: US6917423B2 - Status: Granted - Kind: B2 - Application number: 10/823,902 - Filed: 2004-04-14 - Published: 2004-09-30 - Granted: 2005-07-12 - Earliest claimed: 2002-01-10 - Assignees: ChemImage Corporation - Inventors: Charles W. Gardner, Jr.; John S. Maier; Matthew P. Nelson; Robert C. Schweitzer; Patrick J. Treado; G. Steven Vanni; Juliane Wolfe - Web page: https://patentgrep.com/patent/US6917423B2 ## CPC - G01N 21/6486: {Measuring fluorescence of biological material, e.g. DNA, RNA, cells (G01N21/6428 takes precedence)} - G01J 3/02: Details - G01J 3/0218: {using optical fibers} - G01J 3/2823: {Imaging spectrometer} - G01J 3/44: Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} - G01N 21/31: Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry {(G01N21/72 takes precedence)} - G01N 21/33: using ultraviolet light (G01N21/39 takes precedence) - G01N 21/359: using near infrared light - G01N 21/645: {Specially adapted constructive features of fluorimeters} - G01N 21/6456: {Spatial resolved fluorescence measurements; Imaging} - G01N 21/6458: {Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16)} - G01N 21/65: Raman scattering - G01N 2021/1736: {with two or more light sources} - G01N 2021/6421: {Measuring at two or more wavelengths} - G01N 2021/6423: {Spectral mapping, video display} - G01N 2021/656: {Raman microprobe} - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - G01J: MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY (light sources F21, H01J, H01K, H05B; investigating properties of materials by optical means G01N) ## Abstract Pathogenic microorganisms are detected and classified by spectral imaging of the Raman light scattered by the organisms. ## Claims ### Claim 1 A method for forming an image of an organic object comprising: a) illuminating said object with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological; b) forming an image of said organic object using Raman shifted light from said object; c) analyzing said Raman shifted light image for patterns characteristic of said organic object. ### Claim 2 (depends on 1) A method for forming an image of an object according to claim 1 further comprising passing said Raman shifted light through a filter selected from the group consisting of a FAST fiber array spectral translator, a Fabry Perot angle tuned filter, an acousto-optic tunable filter, a liquid crystal tunable filter, a Lyot filter, and an Evan's split element liquid crystal tunable filter. ### Claim 3 (depends on 1) A method for forming an image of an object according to claim 1 further comprising passing said Raman shifted light through an interferometer selected from the group consisting of a polarization-independent imaging interferometer, a Michelson interferometer, a Sagnac interferometer, a Twynam-Green Interferometer, and a Mach-Zehnder Interferometer. ### Claim 4 (depends on 1) A method for forming an image of an object according to claim 1 further comprising dispersing said Raman shifted light thereby providing spatially separated Raman spectra. ### Claim 5 (depends on 1) A method for forming an image of an object according to claim 1 further comprising providing spatially separated Raman spectra. ### Claim 6 (depends on 1) A method for forming an image of an object according to claim 1 further comprising gathering information from said first image and forming a second image using said information. ### Claim 7 (depends on 6) A method for forming an image of an object according to claim 6 wherein said information comprises a location of said object. ### Claim 8 (depends on 6) A method for forming an image of an object according to claim 6 wherein said information comprises a characterization of said object. ### Claim 9 (depends on 1) A method for forming an image of an object according to claim 1 further comprising forming an image of at least one anthrax spore. ### Claim 10 (depends on 1) A method for forming an image of an object according to claim 1 further comprising forming an image of an object selected from the group consisting of filoviruses, naviruses, alphaviruses. ### Claim 11 (depends on 1) A method for forming an image of an object according to claim 1 further comprising forming an image of an object selected from the group of microorganisms consisting of protozoa, cryptosporidia microorganisms, Escherichia coli, Escherichia coli 157 microorganisms, Plague (Yersinia pestis), Smallpox (variola major), Tularemia (Francisella tularensis), Brucellosis (Brucella species), Clostridium perfringens, Salmonella, Shigella, Glanders (Burkholderia mallei), Melioidosis (Burkholderia pseudomallei), Psittacosis (Chlamydia psittaci), Q fever (Coxiella burnetil), Typhus fever (Rickettsia prowazekii), and Vibrio cholerae. ### Claim 12 (depends on 1) A method for forming an image of an object according to claim 1 further comprising forming an image of an object selected from the group of consisting of Giardia, Candida albicans, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Enterobacter aerogenes, Corynebacterium diphtheriae, Pseudomonas eruginosa, Acinetobacter calcoaceticus, Klebsiella pneumoniae, and Serratia marcescens. ### Claim 13 A method for forming an image of one of a plurality of organic objects comprising: a) providing said plurality of organic objects in a bio-dilute sample; b) illuminating said sample with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological; c) forming an image of one of said plurality of organic objects using Raman shifted light from said imaged organic object; d) analyzing said Raman shifted light image for patterns characteristic of said organic object. ### Claim 14 (depends on 13) A method for forming an image of an object according to claim 13 further comprising passing said Raman shifted light through a filter selected from the group consisting of a FAST fiber array spectral translator, a Fabry Perot angle tuned filter, an acousto-optic tunable filter, a liquid crystal tunable filter, a Lyot filter, and an Evan's split element liquid crystal tunable filter. ### Claim 15 (depends on 13) A method for forming an image of an object according to claim 13 further comprising passing said Raman shifted light through an interferometer selected from the group consisting of a polarization-independent imaging interferometer, a Michelson interferometer, a Sagnac interferometer, a Twynam-Green Interferometer, and a Mach-Zehnder Interferometer. ### Claim 16 (depends on 13) A method for forming an image of an object according to claim 13 further comprising dispersing said Raman shifted light thereby providing spatially separated Raman spectra. ### Claim 17 (depends on 13) A method for forming an image of an object according to claim 13 further comprising providing spatially separated Raman spectra. ### Claim 18 (depends on 13) A method for forming an image of an object according to claim 13 further comprising gathering information from said first image and forming a second image using said information. ### Claim 19 (depends on 18) A method for forming an image of an object according to claim 18 wherein said information comprises a location of said object. ### Claim 20 (depends on 18) A method for forming an image of an object according to claim 18 wherein said information comprises a characterization of said object. ### Claim 21 (depends on 13) A method for forming an image of an object according to claim 13 further comprising forming an image of at least one anthrax spore. ### Claim 22 (depends on 13) A method for forming an image of an object according to claim 13 further comprising forming an image of an object selected from the group consisting of filoviruses, naviruses, alphaviruses. ### Claim 23 (depends on 13) A method for forming an image of an object according to claim 13 further comprising forming an image of an object selected from the group of microorganisms consisting of protozoa, cryptosporidia microorganisms, Escherichia coli, Escherichia coli 157 microorganisms, Plague (Yersinia pestis), Smallpox (variola major), Tularemia (Francisella tularensis), Brucellosis (Brucella species), Clostridium perfringens, Salmonella, Shigella, Glanders (Burkholderia mallei), Melioidosis (Burkholderia pseudomallei), Psittacosis (Chlamydia psittaci), Q fever (Coxiella burnetii), Typhus fever (Rickettsia prowazekii), and Vibrio cholerae. ### Claim 24 (depends on 13) A method for forming an image of an object according to claim 13 further comprising forming an image of an object selected from the group of consisting of Giardia, Candida albicans, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Enterobacter aerogenes, Corynebacterium diphtheriae, Pseudomonas eruginosa, Acinetobacter calcoaceticus, Klebsiella pneumoniae, and Serratia marcescens. ### Claim 25 A method for forming an image of one of a plurality of pathogenic microorganisms comprising: a) providing a bio-dilute sample of said plurality of pathogenic microorganisms; b) illuminating said bio-dilute sample with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological; c) forming an image of one of said plurality of pathogenic microorganisms using Raman shifted light from said imaged pathogenic microorganism; d) analyzing said Raman shifted light image for patterns characteristic of said one of said plurality of pathogenic microorganisms. ### Claim 26 A method for forming an image of an organic object comprising: a) illuminating a biodilute sample including said organic object with light in the absence of a bioconcentrator which is capable of forming a complex between an analyte and a biological; b) forming an image of said illuminated organic object using Raman shifted light from said object; c) analyzing said Raman shifted light image for patterns characteristic of said organic object.