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US 6,916,665 B2Granted
Biosensor compositions and methods of use
AssigneeThe Texas A&M University System
InventorsHagan P. Bayley, Stefan G. Howorka, Liviu Movileanu
Life of this patent
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Abstract
Provided are pore-subunit polypeptides covalently linked to one or more sensing moieties, and uses of these modified polypeptides to detect and/or measure analytes or physical characteristics within a given sample.
Claims14
A method of detecting the presence of an analyte in a sample, the method comprising:
- contacting said sample with a pore assembly comprising one or more pore-subunit polypeptides sufficient to form a pore, wherein the pore comprises at least a first channel, and at least one of said pore-subunit polypeptides is a modified pore-subunit polypeptide comprising a pore-subunit polypeptide covalently linked to an exogenous sensing moiety capable of preferentially binding with a specific analyte;
- and
- detecting an electrical current through at least a first channel, wherein a modulation in current compared to a current measurement in a control sample lacking said analyte indicates the presence of said analyte in said sample.
A method of detecting the presence of an analyte in a sample, wherein the analyte comprises a polynucleic acid comprising a specific base sequence, the method comprising:
- contacting said sample with a pore assembly comprising one or more pore-subunit polypeptides sufficient to form a pore, wherein the pore comprises at least a first channel, and at least one of said pore-subunit polypeptides is a modified pore-subunit polypeptide comprising a pore-subunit polypeptide covalently linked to an exogenous sensing moiety that is an oligonucleotide, wherein the oligonucleotide comprises a base sequence that is complementary to said specific base sequence of said analyte;
- and
- detecting an electrical current through at least a first channel, wherein a modulation in current compared to a current measurement in a control sample lacking said analyte indicates the presence of said analyte in said sample.
Dependencies are inferred from the claim text.