Back to results
US 6,916,663 B2Granted
Detection method of proteins on polyacrylamide gels using a dye composition as a silver ion sensitizing agent and the dye composition for the same
AssigneesEZ BioPaQ Co., Ltd., San-Ock Jeong
InventorJung-Kap Choi
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for detecting proteins on polyacrylamide gels, comprising the steps of sensitizing the proteins with a dye composition comprising an acidic organic dye as a silver ion sensitizing agent, and optionally, a basic organic dye as an auxiliary agent, and washing them, and then silver staining complexes of the protein-dye, and to the dye composition for the same.
Claims8
A method for detecting proteins on polyacrylamide gels, comprising the steps of:
- (a) sensitizing the proteins with a dye composition to form a protein dye complex, containing one or more acidic organic dyes selected from the group consisting of calcon dye derivatives, alizarin yellow GG, alizarin yellow R, zincon, Bordeaux R, tryphan blue, and acid blue 92 as a silver ion sensitizing agent, and a basic organic dye selected from the group consisting of brilliant cresyl blue ALD, direct blue 71, crystal violet, methyl violet B base, methyl violet 10B, methyl violet B, ethyl green, rhodamine B, ethyl violet, phenosafranine and methylene blue as an auxiliary agent;
- and then, washing them;
- (b) impregnating silver ions to complexes of the protein-dye, and then, reducing them to metallic silver;
- and
- (c) observing the protein-dye-metallic silver complexes.
2 more elements
Dependencies are inferred from the claim text.