Virus envelope vector for gene transfer
AssigneeAnGes MG, Inc.
InventorYasufumi Kaneda
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Abstract
A gene transfer vector is prepared by introducing an exogenous gene into an inactivated virus envelope, through a freezing and thawing treatment or mixing with a detergent. There are also provided a pharmaceutical composition for gene therapy containing this gene transfer vector, a kit containing this gene transfer vector, and a gene transfer method employing this gene transfer vector.
Claims7
A gene transfer vector comprising an exogenous gene encapsulated in a native virus envelope, prepared by a method comprising the steps of:
- adding protamine sulfate to the exogenous gene;
- mixing a virus with the exogenous gene;
- and
- freezing and thawing the mixture two or more times.
A method for preparing a gene transfer vector comprising an exogenous gene encapsulated in a native virus envelope for gene transfer, wherein the method comprise the steps of:
- mixing a virus with the exogenous gene;
- freezing and thawing the mixture two or more times;
- and
- inactivating the virus.
A method for introducing an exogenous gene into a suspended cell, wherein the method comprises the steps of:
- mixing the suspended cell with a gene transfer vector comprising the exogenous gene encapsulated in a native virus envelope in the presence of protamine sulfate;
- and
- centrifuging the mixture.
A gene transfer vector comprising an exogenous gene encapsulated in a native virus envelope, wherein the gene transfer vector is prepared by a method comprising the steps of:
- adding protamine sulfate to the exogenous gene;
- mixing a virus with the exogenous gene in the presence of a detergent.
Dependencies are inferred from the claim text.