PEG-modified uricase
AssigneePhoenix Pharmacologics, Inc.
InventorsCharles Mark Ensor, Mike A. Clark, Frederick Wayne Holtsberg
Life of this patent
Estimating the term…
Abstract
The present invention is directed to uricase modified with polyethylene glycol and to methods of treating different illnesses characterized by increased circulating uric acid levels, including but not limited to, hyperuricemia and tumor lysis syndrome.
Claims29
A compound comprising uricase covalently bonded via a linking group to polyethylene glycol, wherein the polyethylene glycol has a total weight average molecular weight of about 12,000 to about 30,000, wherein the linking group is selected from the group consisting of a succinimide group, an amide group, an imide group, a carbamate group, an ester group, an epoxy group, a carboxyl group, a hydroxyl group, a carbohydrate, a tyrosine group, a cysteine group, a histidine group and combinations thereof and wherein said uricase comprises 12 to about 30 polyethylene glycol molecules per uricase protein unit.
A method of enhancing the circulating half life of uricase comprising modifying said uricase by covalently bonding said uricase via a linking group to polyethylene glycol, wherein the polyethylene glycol has a total weight average molecular weight of about 12,000 to about 30,000, wherein said uricase comprises 12 to 30 polyethylene glycol molecules per uricase protein unit, and wherein the linking group is selected from the group consisting of a succinimide group, an amide group, an imide group, a carbamate group, an ester group, an epoxy group, a carboxyl group, a hydroxyl group, a carbohydrate, a tyrosine group, a cysteine group, a histidine group and combinations thereof.
A compound comprising uricase coupled to polyethylene glycol, wherein the polyethylene glycol has a total weight average molecular weight of about 12,000 to about 30,000 and wherein said uricase comprises 12 to about 30 polyethylene glycol molecules per uricase protein unit.
Dependencies are inferred from the claim text.