Irreversible immobilization of enzymes into polyurethane coatings

A method of irreversibly immobilizing diisopropylfluorophosphatase into polyurethane coatings comprising the steps of:

• reacting a mixture of a polyol dispersion coreactant having a water content of 70 w. %, a polyether modified polydimethyl siloxane surfactant, a buffered medium comprised of bis-tris-propane buffer and CaCl2 and diisopropylfluorophosphatase to create an aqueous mixture;
• adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
• applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating;
• and
• curing the enzyme-containing polyurethane coating.

An enzyme-containing composition made by the process comprising the steps of:

• reacting a mixture of a polyol dispersion coreactant and an enzyme to create an aqueous mixture;
• adding a water-dispersible aliphatic polyisocyanate based on hexamethylene diisocyanate to the aqueous mixture in an amount sufficient to produce an emulsion capable of forming a polyurethane coating;
• applying the emulsion onto thermoplastic polyolefin panels to create an enzyme-containing polyurethane coating;
• and
• curing the enzyme-containing polyurethane coating.