Method for modulating steroidogenic activity
AssigneeNational University of Singapore
InventorsYek Cheng Ong, Eu Leong Yong
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Abstract
The present invention relates generally to a method for modulating steroidogenic activity and a composition useful for same. The present invention further relates to a composition comprising a steroidogenic modulator useful for modulating physiological processes mediated by the androgen receptor or an active form thereof or complex comprising same and/or for modulating physiological processes mediated by estrogen receptors. The composition of the present invention preferably comprises an extract of herbs or botanical or horticultural equivalents of the herbs or chemical or functional equivalents of one or more components of the herbal extract thereof.
Claims18
A composition comprising:
- a preparation of Eucommia ulmoides prepared by ethanol extraction thereof and chromatography a) over a hydroxypropylated, cross-linked dextran matrix having an exclusion limit for peptides of 4 kilodaltons and an exclusion limit for small organic molecules of 5 kilodaltons with elution using n-butanol or b) over a reverse phase matrix with elution by an aqueous buffer and acetonitrile gradient or c) over a diol matrix with elution using at least one organic solvent;
- that lacks fluorescent components exhibiting Rf values of 0 and 0.4 and also lacks vanillin-sulphuric acid reactive components exhibiting Rf values 0.47, 0.62, 0.71 and 1 when the composition is separated by two dimensional thin layer chromatography on silica gel and eluted in the first dimension with dichioromethane and in the second dimension with hexane.
Dependencies are inferred from the claim text.