Methods of treating HIV infected subjects

A method for inducing ex vivo proliferation of a population of T cells from an HIV-infected subject to sufficient numbers for use in therapy, comprising contacting a population of T cells ex vivo with a surface having attached thereto:

• an anti-CD3 antibody or CD3-binding fragment thereof, which stimulates a TCR/CD3 complex-associated signal in T cells, and
• an anti-CD28 antibody or CD28-binding fragment thereof, which stimulates a CD28-sssociated signal in T cells;
• wherein the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, are attached on the same surface,
• the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, thereby inducing the T cells to proliferate to sufficient numbers for use in therapy;
• and
• whereby the percent CD4+ T cells expressing the cell surface marker CD45RO increases.

A method of treating an HIV-infected subject, comprising contacting a population of T cells from the subject ex vivo with a surface having attached thereto:

• an anti-CD3 antibody or CD3-binding fragment thereof, which stimulates a TCR/CD3 complex-associated signal in T cells, and
• an anti-CD28 antibody or CD28-binding fragment thereof, which stimulates a CD28-sssociated signal in T cells;
• wherein the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, are attached on the same surface,
• the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, thereby inducing the T cells to proliferate to sufficient numbers for use in therapy;
• whereby the CD28 stimulation by the anti-CD28 antibody or CD28-binding fragment thereof provides a proliferative advantage for HIV-iminfected CD4+ T cells over HIV-infected CD4+ T cells and a protective effect against HIV infection for uninfected CD4+ T cells, and readministering the T cells to the subject.

A method of treating an HIV-infected subject, comprising contacting a population of T cells from the subject ex vivo with a surface having attached thereto:

• an anti-CD3 antibody or CD3-binding fragment thereof, which stimulates a TCR/CD3 complex-associated signal in T cells, and
• an anti-CD28 antibody or CD28-binding fragment thereof, which stimulates a CD28-sssociated signal in T cells;
• wherein the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, are attached on the same surface,
• the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, thereby inducing the T cells to proliferate to sufficient numbers for use in therapy;
• whereby the percent of CD4+ T cells expressing the cell surface marker CD45RO increases, and readministering the T cells to the subject.