Factor VII glycoforms
AssigneeNovo Nordisk Health Care A/S
InventorsHans Kurt Pingel, Niels Kristian Klausen
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Abstract
The present invention provides preparations of Factor VIIa polypeptides or Factor VIIa-related polypeptides that exhibit predetermined glycoform patterns. The preparations of the invention exhibit improved functional properties and are useful for treating Factor VII-mediated conditions.
Claims27
A preparation comprising a plurality of Factor VII polpeptides or Factor VII-related polypeptides produced in Chinese Hamster Ovary-K1 (CHO-K1) cells in the absence of serum, wherein said cells are adapted to grow in the absence of serum and are cultured in the absence of serum both in the growth phase and in the production phase.
A method for producing a prepararation comprising Factor VII polypeptides or Factor VII-related polypeptides having a predetermined pattern of N-linked glycosylation, said method comprising culturing a Chinese Hamster Ovary-K1 (CHO-K1) cell that has been adapted to grow in the absence of serum in a medium lacking serum for both growth and production phases.
A preparation comprising a plurality of Factor VII polypeptides or Factor VII-related polypeptides produced in Chinese Hamster Ovary-K1 (CHO-K1) cells in the absence of animal-derived components, wherein said cells are adapted to grow in the absence of animal-derived components and are cultured in the absence of animal-derived components both in the growth phase and in the production phase.
A method for producing a preparation comprising Factor VII polypeptides or Factor VII-related polypeptides having a predetermined pattern of N-linked glycosylation, said method comprising culturing a Chinese Hamster Ovary-K1 (CHO-K1) cell that has been adapted to grow in the absence of animal-derived components in a medium lacking animal-derived components for both growth and production phases.
Dependencies are inferred from the claim text.