Chemically defined medium for cultured mammalian cells

A chemically defined medium, said medium suitable for adaptation and growth of immortalized mammalian cells in culture to high cell densities, said medium comprising, sodium chloride, 3-5 g/L;

• potassium chloride, 0.2-0.4 g/L;
• , HEPES, 5-7 g/L;
• glucose (dextrose), 3.5-5.5 g/L;
• biotin, 0.000005-0.000025 g/L;
• ascorbic acid, 0.002-0.004 g/L;
• pantothenate, 0.002-0.006 g/L;
• choline, 0.002-0.006 g/L;
• folate, 0.002-0.006 g/L;
• inositol, 0.005-0.02 g/L;
• niacinamide, 0.002-0.006 g/L;
• pyridoxal, 0.002-0.006 g/L;
• riboflavin, 0.0002-0.0006 g/L;
• thiamine, 0.002-0.006 g/L;
• cyanocbalamin, 0.000005-0.000025 g/L;
• oxaloacetic acid, 0.1-0.4 g/L;
• alanine, 0.015-0.035 g/L;
• asparagine, 0.01-0.035 g/L;
• arginine, 0.06-0.10 g/L;
• aspartate, 0.02-0.04 g/L;
• cysteine, 0.3-0.5 g/L;
• cystine, 0.05-0.2 g/L;
• glutamine, 0.8-1.5 g/L;
• glutamate, 0.06-0.09 g/L;
• glycine, 0.02-0.04 g/L;
• histidine, 0.03-0.05 g/L;
• isoleucine, 0.05-0.25 g/L;
• leucine, 0.05-0.25 g/L;
• lysine, 0.05-0.25 g/L;
• methionine, 0.02-0.04 g/L;
• phenylalanine, 0.055-0.075 g/L;
• proline, 0.03-0.05 g/L;
• serine, 0.03-0.55 g/L;
• threonine, 0.07-0.15 g/L;
• tryptophan, 0.005-0.025 g/L;
• tyrosine, 0.05-0.15 g/L;
• valine, 0.094 g/L;
• sodium selenate, 0.0000005-0.000060 g/L;
• magnesium sulfate, 0.05-0.2 g/L;
• potassium chloride, 0.15-0.45 g/L;
• sodium phosphate, 0.075-0.2 g/L;
• potassium nitrate, 0.00005-0.00009 g/L;
• calcium chloride, 0.08-0.25 g/L;
• sodium pyruvate 0.05-0.4 g/L;
• insulin, 0.05-2 g/L;
• hydrocortisone, 20-80 μg/L;
• linoleic acid, 1-100 mg/L;
• ethanolamine, 5-25 μg/L;
• sodium bicarbonate, 1-5 g/L;
• APO transferrin or ferric citrate, 1-10 mg/L;
• Pluronic F68, 0.2-2 g/L;
• sodium hydroxide, 0.3-0.9 g/L;
• mycophenolic acid, 0.1-2 mg/L;
• hypoxanthine, 2-5 mg/L;
• xanthine;
• 10-200 mg/L;
• sodium bicarbonate 1.5-4.5 g/L.