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AssigneesPresident and Fellows of Harvard College, The General Hospital Corporation, Forsyth Dental Infirmary for Children
InventorsPamela C. Yelick, John D. Bartlett, Joseph P. Vacanti, Bjorn R. Olsen, Phillip Stashenko
Estimating the term…
Tooth tissues include the pulp mesenchyme that forms the dentin and an epithelium that is responsible for enamel formation. Cells from these tissues were obtained from porcine third molars and were seeded onto a biodegradable scaffold composed of a polyglycolic acid—polylactic acid copolymer. Cell polymer constructs were then surgically implanted into the omentum of athymic nude rats so that the constructs would have a blood supply and these tissues were allowed to develop inside the rats. Infrequently, columnar epithelial cells were observed as a single layer on the outside of the dentin-like matrix similar to the actual arrangement of ameloblasts over dentin during early tooth development. Developing tooth tissues derived from such cell polymer constructs could eventually be surgically implanted into the gum of an edentulous recipient where the construct would receive a blood supply and develop to maturity, providing the recipient with a biological tooth replacement.
A method to make new tooth tissue comprising, applying tooth germ cells onto a biodegradable polymer scaffold and allowing the tooth germ cells to develop into a tooth for implantation, wherein the tooth germ cells comprise cells from an enamel organ and a pulp organ.
A method of generating tooth tissue comprising, applying tooth germ cells onto a biodegradable polymer scaffold and allowing the tooth germ cells to develop into a tooth for implantation, wherein the tooth germ cells comprise cells dissociated from an enamel organ, a pulp organ, and from tissue cultured cells derived from tooth tissues.
A method of generating tooth tissue comprising, applying tooth germ cells onto a biodegradable polymer scaffold and allowing the tooth germ cells to develop into a tooth for implantation, wherein the scaffold is implanted into an omentum of a host animal.
A The method of generating tooth tissue comprising, forming a biodegradable polymer scaffold, applying tooth germ cells onto the biodegradable polymer scaffold, and implanting the scaffold into a host animal, wherein the tooth germ cells comprise cells dissociated from an enamel organ and a pulp organ.
A method of generating tooth tissue comprising preparing a tooth mold in the shape of a human tooth, forming a biodegradable polymer scaffold in the tooth mold, applying tooth germ cells onto the biodegradable polymer scaffold, and implanting the scaffold into an omentum of a host animal.
Dependencies are inferred from the claim text.