System and method for electroporating a sample
AssigneeAmbion, Inc.
InventorsRichard A. Jarvis, Mike W. Byrom, Dmitriy Ovcharenko
Life of this patent
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Abstract
A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.
Claims40
A system for electroporating a sample comprising:
- a housing having a lower body and an upper body;
- a first removable electrode having a first sample contact surface, the first removable electrode coupled to the lower body;
- a second removable electrode having a second sample contact surface, the second removable electrode coupled to the upper body;
- the first removable electrode and the second removable electrode positioned operable to confine a surface tension constrained sample between the first sample contact surface and the second sample contact surface;
- and
- the first removable electrode and the second removable electrode operable to receive an electric pulse from a pulse generator.
An electrode array comprising:
- an electrode base operable to conductively couple to an electroporation body;
- the electrode base having a plurality of sample contact surfaces disposed thereon, the contact surfaces sized to hold an electroporation sample;
- and
- the plurality of sample contact surfaces operable to receive an electric pulse from a pulse generator;
- and
- the plurality of sample contact surfaces sized and disposed on the electrode base to correspond with a plurality of sample contact surfaces associated with a corresponding electrode array.
A system for electroporating a sample comprising:
- a housing having a lower body and an upper body;
- a first electrode array having a plurality of lower sample contact surfaces, the first electrode array coupled to the lower body;
- a second electrode array having a plurality of upper sample contact surfaces corresponding to the plurality of lower sample contact surfaces, the second electrode array coupled to the upper body;
- the lower body and the upper body positioned operable to confine a surface tension constrained sample between each of the plurality of lower sample contact surfaces and each of the plurality of upper sample contact surfaces;
- and
- the first electrode array and the second electrode array operable to receive an electric pulse from a pulse generator.
A method for electroporating a sample comprising:
- positioning an upper body of an electroporation system in an access position;
- depositing a sample on a sample contact surface of a first electrode disposed in the lower body of the electroporation system;
- positioning the upper body in an operational position such that the sample is confined between the lower contact surface and an upper contact surface of a second electrode disposed in the upper body;
- and
- sending an electric pulse between the lower electrode and the upper electrode.
Dependencies are inferred from the claim text.