Heat-resistant mannose isomerase, process for producing the same and process for producing mannose by using the same
AssigneeJapan Science and Technology Corporation
InventorYoshiyuki Takasaki
Life of this patent
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Abstract
A thermostable mannose isomerase generated from a bacterium belonging to the genus Agrobacterium, excellent in thermostability, capable of being continuously used at a temperature of 55° C. to 60° C. for a prolonged period of time, and has, for example, the following enzymatic properties: (a) function: converts D-mannose to D-fructose and vice versa; (b) substrate specificity: isomerizes the aldoses D-mannose and D-lyxose to their corresponding kitoses, but does not interact with D-glucose, D-galactose, L-mannose, D-xylose, L-xylose, D-arabinose, L-arabinose or D-ribose; (c) optimum pH: 7.5 to 8.5; (d) optimum temperature: 55° C. to 60° C.; and (e) stable pH: 6.0 to 10.0, is provided.
Claims4
A thermostable mannose isomerase, which is generated from a bacterium belonging to the genus Agrobacterium, which thermostable mannose isomerase shows the following enzymatic properties:
- (a) function:
- converts D-mannose to D-fructose and vice versa;
- (b) substrate specificity:
- isomerizes the aldoses D-mannose and D-lyxose to their corresponding ketoses;
- however, does not function with D-glucose, D-galactose, L-mannose, D-xylose, L-xylose, D-arabinose, L-arabinose or D-ribose;
- (c) optimum pH:
- 7.5 to 8.5;
- (d) optimum temperature:
- continuously for two to three months at a temperature of 60° C.;
- and
- (e) stable pH:
- 6.0 to 10.0.
Dependencies are inferred from the claim text.