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AssigneeGangaGen, Inc.
InventorsJanakiraman Ramachandran, Sriram Padmanabhan, Bharathi Sriram
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The present invention features therapeutic bacteriophage deficient in the lysin protein (“Lys minus” phage). Lys minus bacteriophage are incapable of facilitating efficient lysis of the bacterial host since the enzymatic activity of the lysin of the phage is needed for breaking down the peptidoglycan layer of the bacterial cell wall. Lys minus bacteriophage retain activity in invasion of its appropriate bacterial host, destruction of the bacterial genome, and replication, which are sufficient to inhibit bacterial growth and replication. Therefore, the therapeutic Lys minus phage stops the spread of infection by the bacterial pathogen without lysis of the bacterium. This approach is attractive as it also prevents the release of the phage progeny, thus reducing or eliminating the potential for generation of immune responses against the phage. The incapacitated bacterial pathogen is then removed by the normal defense systems such as phagocytes and macrophages.
A method for inhibiting growth of bacteria in an infected subject, the method comprising:
A method for treating a bacterial infection in an infected subject, the method comprising:
A method for treating a bacterial infection in an infected subject with a therapeutic bacteriophage so as to provide for reduced bacteriophage clearance by the subject's immune system, the method comprising:
A pharmaceutical composition comprising a Lys minus bacteriophage present in an amount effective to inhibit growth of bacterium in an infected subject and a pharmaceutically acceptable carrier, where upon contacting a bacterial host cell, the Lys minus bacteriophage effects inhibition of growth of the bacterial host cell in the infected subject.
An isolated Lys minus bacteriophage, which bacteriophage is defective in production of a functional lysin protein, wherein the Lys minus bacteriophage is capable of infecting a bacterial host cell and inhibiting replication by the infected host cell without causing significant lysis of the host cell by virtue of the action of bacteriophage lysis system.
Dependencies are inferred from the claim text.