Transgenic non-human animals expressing a truncated activintype II receptor

A method of producing a chimeric non-human mammal, the method comprising:

• obtaining an ovum from ovaries of a non-human mammal;
• maturing the ovum in vitro;
• fertilizing the mature ovum in vitro to form a zygote;
• introducing into the zygote in vitro a nucleic acid construct comprising in operable association a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a regulatory element comprising a muscle-specific promoter that promotes expression of the DNA sequence encoding the truncated dominant negative Activin Type II receptor;
• maturing the zygote to a preimplantation stage embryo in vitro;
• and
• transplanting the embryo into a recipient female mammal of the same species, wherein the female mammal gestates the embryo to produce a chimeric animal.

A method of producing food products from a transgenic non-human mammal having increased muscle mass comprising:

• a) introducing a transgene comprising in operable association a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a regulatory element comprising a muscle-specefic promoter into germ cells of a pronuclear embryo of the mammal;
• b) implanting the embryo into the oviduct of a pseudopregnant female of the same species, thereby allowing the embryo to mature to full term progeny;
• c) testing the progeny for presence of the transgene to identify transgene-positive progeny;
• d) cross-breeding transgene-positive progeny to obtain further transgene-positive progeny;
• and
• e) processing the progeny to obtain food products.

A method of producing food products from a transgenic ovine, porcine, or bovine mammal having increased muscle mass comprising:

• a) introducing a transgene comprising in operable association a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a regulatory element comprising a muscle-specific promoter into an embryo of an ovine, porcine, or bovine mammal;
• b) implanting the embryo into the oviduct of a pseudopregnant female of the same species, thereby allowing the embryo to mature to full term progeny;
• c) testing the progeny for presence of the transgene to identify transgene-positive progeny;
• d) cross-breeding transgene-positive progeny to obtain a transgenic ovine, porcine, or bovine mammal;
• and
• e) processing the transgenic mammal to obtain food products.

A method of producing a chimeric non-human mammal, the method comprising:

• obtaining an ovum from ovaries of a non-human mammal;
• maturing the ovum in vitro;
• fertilizing the mature ovum in vitro to form a zygote;
• introducing into the zygote in vitro a nucleic acid construct comprising in operable association a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a myosin light chain promoter and ⅓ enhancer, which promotes expression of the DNA sequence encoding the truncated dominant negative Activin Type II receptor;
• maturing the zygote to a preimplantation stage embryo in vitro;
• and
• transplanting the embryo into a recipient female mammal of the same species, wherein the female mammal gestates the embryo to produce a chimeric animal.

A method of producing food products from a transgenic non-human mammal having increased muscle mass comprising:

• a) introducing a transgene comprising in operable associate a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a myosin light chain promoter and ⅓ enhancer, into germ cells of a pronuclear embryo of the mammal;
• b) implanting the embryo into the oviduct of a pseudopregnant female of the same species, thereby allowing the embryo to mature to full term progeny;
• c) testing the progeny for presence of the transgene to identify transgene-positive progeny;
• d) cross-breeding transgene-positive progeny to obtain further transgene-positive progeny;
• and
• e) processing the progeny to obtain food products.

A method of producing food products from a transgenic ovine, porcine, or bovine mammal having increased muscle mass comprising:

• a) introducing a transgene comprising in operable associate a DNA sequence encoding a truncated dominant negative Activin Type II receptor, which lacks kinase activity, and a myosin light chain promoter and ⅓ enhancer into an embryo of an ovine, porcine, or bovine mammal;
• b) implanting the embryo into the oviduct of a pseudopregnant female of the same species, thereby allowing the embryo to mature to full term progeny;
• c) testing the progeny for presence of the transgene to identify transgene-positive progeny;
• d) cross-breeding transgene-positive progeny to obtain a transgenic ovine, porcine, or bovine mammal;
• and
• e) processing the tranagenic mammal to obtain food products.