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US 6,887,986 B1Granted
α-amylase variants
AssigneeNovozymes A/S
InventorsAllan Svendsen, Soren Kjaerulff, Henrik Bisgard-Frantzen, Carsten Andersen
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AssigneeNovozymes A/S
InventorsAllan Svendsen, Soren Kjaerulff, Henrik Bisgard-Frantzen, Carsten Andersen
Estimating the term…
The invention relates to a variant of a parent Termamyl-like α-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an α-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an α-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an α-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an α-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like α-amylase, which variant exhibits increased.
A DNA sequence encoding a variant of a parent Termamyl-like alpha-amylase, said variant having an amino acid sequence which is at least 80% identical to SEQ ID NO:4, having alpha-amylase activity and comprising an alteration at a position corresponding to position 356 in SEQ ID NO:4.
A DNA sequence encoding a variant of a parent Termamyl-like alpha-amylase, said variant having an amino acid sequence which is at least 80% identical to SEQ ID NO:4, having alpha-amylase activity and comprising an alteration of E376K, using SEQ ID NO:4 for numbering.
Dependencies are inferred from the claim text.